Advancements in Two-Dimensional Liquid Chromatography for Enhanced Food and Environmental Analysis

Arshdeep Chopra*

Two-dimensional liquid chromatography (2D-LC) represents a significant advancement in food analysis, addressing the limitations of one-dimensional liquid chromatography (1D-LC) in separating complex food matrices. Food analysis plays a critical role in assessing the components, physicochemical properties, sensory qualities, and contamination risks of food products. Given the rising interest in identifying bioactive compounds in food due to their health benefits, the need for enhanced analytical techniques has become more pressing. Traditional 1D-LC often struggles to resolve closely related and co-eluting species in complex food samples, necessitating methods with greater resolving power. 2D-LC offers a valuable alternative by coupling two independent separation processes, thereby significantly increasing separation efficiency. This approach is particularly effective when paired with mass spectrometry (MS), which enhances detection sensitivity and specificity, allowing for more accurate structural identification. Moreover, 2D-LC has proven instrumental in environmental analysis, particularly in detecting and characterizing organic micropollutants (OMPs) at trace levels. As the demand for comprehensive food analysis and environmental monitoring grows, 2D-LC coupled with MS is poised to play an increasingly vital role in ensuring food safety, quality, and informed consumer choices. Therefore, this review briefly introduces the working principle of 2D-LC concerning its application in the field of food and environmental analysis in recent years.

Graphical abstract: Various applications of 2D-LC in food and environmental analysis.

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Published on: Mar 10, 2026 Pages: 1-14

Full Text PDF Full Text HTML DOI: 10.17352/ojabc.000034
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